WebThe DyLight Dyes have absorption spectra ranging from 350 nm to 770 nm (Table 1). These reagents fluoresce across a broad pH range with excitation wavelengths that … WebTable 2. Properties of the Thermo Scientific DyLight Dyes. DyLight Dye A. max * ε† CF ‡ 350 . 405 . 488 . 550 . 594 . 633 . 650 . 680 . 755 . 800 * Excitation wavelength in nanometers − note that upon protein conjugation the absorption maximum shifts to the right of the spectra †Molar extinction coefficient (M-1. cm-1) at A. max ...
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WebJan 1, 2007 · The DyLight™ Dy es exhibit absorption spectra ranging from 493 nm to 770 nm and offer . ... New laser diodes emitting near 650 nm should also be excellent excitation sources for Cy5. View. WebIHC-P. 1/50 - 1/500. DyLight ® 650 replaces DyLight ® 649. The DyLight ® 650 excitation is 652nm and the emission is 672nm. DyLight ® 650 hence provides the same far-red fluorescence and photostability as the DyLight ® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 649 or DyLight ® 650. danish micro engineering a/s
Cytek Full Spectrum Viewer
WebCells were probed with a DyLight 650-conjugated Myc Epitope Tag monoclonal antibody (Product # MA1-21316-D650) at a dilution of 1:25 for at least 1 hour at room temperature. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). WebSpectra Viewer . Dyes similar to DyLight 649 based on emission ... Plum CF647 CF647ST CF650 CF660ST CoraLite Plus 647 CPN680 Red Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DRAQ 5 DY-520XL DY-521XL DyLight 650 Dyomics 647 eFluor 660 eFluor 670 Cell Proliferation ER-ID Red FluoProbes647H FVS660 HiLyte 647 iFluor 647 IRDye … Webd2 and Green dye excitation spectra overlap Lumi4-Tb cryptate emission (Eu3+ Cryptate as well for d2 acceptor only), thereby allowing the donor to excite its acceptor, whose maximum emission at 665 nm (d2) or 520 nm (Green) spans a region where HTRF cryptates do not emit or do so only weakly. ... 650 nm: 653 nm: 488 nm: Emission: 660 nm Short ... danish mall shooting